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KMID : 0380019940090020191
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 2 p.191 ~ p.198
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Optimum Conditions for the Protoplast Formation of Lactobacillus plantarum and Leuconostoc mesenteroides
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Abstract
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Protoplasts of both strains were produced by lysozyme digestion at 301C for 180 min. Both strains were treated with 40ug/ml of lysozyme in 30mM Tris-HC1 buffer(pH 7.5) containing 10% sucrose at the late logarithmic growth phase. It was found that the efficiency of protoplast formation was high at 301C and pH 7.5 by measuring the decrease in absorbance. Optimum concentrations of sucrose Ca", Mg" for protoplast formation were determined to be 15%, 20mM and 6mM, respectively. Hydrolysis of cell wall and protoplast formation efficiency for L. plantarum showed better results than those for Leu. mesenteroides. The resistances to antibiotics erythromycin and chloramphenicols were chosen as the selection marker for the fusant between L. plantarum and Leu. mesenteroides. Production phase of protoplast in Leu. mesenteroides was also compared with L. plantarum in this paper.
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